Quality-assured methods multi-species surveys (Metabarcoding)
As the first provider in Sweden, we now have a fully developed and quality-assured process. From water sampling to results that show the complete diversity of species of fish, amphibians and river mussels. We have performed more than 20 projects based on metabarcoding with good results. During method development and verification of the fish metabarcoding methodology, we found that all species that had been detected using exploratory fishing over 50 years in 40 sampled lakes had been detected using eDNA. Further, we found new species in some of the lakes that could also be subsequently verified. The method is highly reliable in describing the species composition of fish and works equally well in the ocean, standing and running water. We also have very good experience of metabarcoding of amphibian communities in ponds and river mussels (Unioids) in lakes and running water. Within a study we compared traditional inventory methods for amphibians with eDNA sampling, and eDNA came out better.
Quality-assured methods for single species analysis
We have also fully developed well-functioning processes to analyse species individually. This concerns e.g. pike, eel, great crested newt, otter, European crayfish, amphibian chytrid fungus and several other species. Concerning species that have not previously been analysed by AquaBiota an additional start-up fee is required, as additional preparations are needed for the genetic work. This method is less costly but provides less information.
As development projects, we offer to test new investigation methods, or completely new approaches, based on the client’s needs or within funded research projects. These development projects are commonly carried out in collaboration with research institutes working with eDNA, with the goal that they will result in new quality-assured methods. Currently we are developing methods for analysing individuals of lynx and polar bear based on samples from snow tracks. We are collaborating with others on development of a Swedish and European standard for eDNA sampling of fish communities.